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Bifidobacterium breve and Streptococcus thermophilus secretion products enhance T helper 1 immune response and intestinal barrier in mice.
- Source :
-
Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2005 Nov; Vol. 230 (10), pp. 749-56. - Publication Year :
- 2005
-
Abstract
- Lactic acid bacteria or their secretion products can modulate immune responses differently in normal and inflammatory conditions. This comparative study analyzes the effect of oral administration of living lactic acid bacteria, or their conditioned media, on the epithelial and immune functions of colitis-prone C57BL/6 IL-10-deficient mice. Mice were untreated (control) or infected with Helicobacter hepaticus with or without oral treatment with living bacteria, Bifidobacterium breve C50 and Streptococcus thermophilus 065 (LB), or their culture-conditioned media (CM). Histology, cytokine mRNA, electrical resistance, and barrier capacity of colonic samples as well as cytokine secretion by mesenteric lymph node (MLN) cells were studied. Helicobacter hepaticus mice developed only mild colitis, which was not modified in LB or CM groups. In the CM (but not the LB) group, the colonic barrier was reinforced as compared to the other groups, as evidenced by decreased horseradish peroxidase (HRP) transcytosis and mannitol fluxes and increased electrical resistance. In MLN, the percentage of CD4+ and CD8+ T cells secreting IFNgamma was significantly higher in CM (2.06% and 1.98%, respectively) mice than in H. hepaticus (1.1% and 0.47%, P < 0.05) or control mice. In addition, the nonspecific stimulation of IFNgamma, TNFalpha, and IL-12 secretion by MLN cells was significantly higher in the CM group as compared to the other groups. In the absence of severe colitis, Bifidobacterium breve C50- and Streptococcus thermophilus 065-conditioned media can reinforce intestinal barrier capacity and stimulate Th1 immune response, highlighting the involvement of lactic acid bacteria-derived components in host defense.
- Subjects :
- Animals
Cells, Cultured
Cytokines analysis
Cytokines metabolism
Enzyme-Linked Immunosorbent Assay
Escherichia coli immunology
Escherichia coli Infections immunology
Escherichia coli Infections pathology
Female
Flow Cytometry
Helicobacter Infections immunology
Helicobacter Infections pathology
Helicobacter hepaticus immunology
Intestines cytology
Intestines immunology
Lymph Nodes cytology
Mice
Mice, Inbred C57BL
Reverse Transcriptase Polymerase Chain Reaction
Bifidobacterium metabolism
Intestinal Mucosa immunology
Streptococcus thermophilus metabolism
Th1 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3702
- Volume :
- 230
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Experimental biology and medicine (Maywood, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 16246902
- Full Text :
- https://doi.org/10.1177/153537020523001008