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Functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2005 Dec 15; Vol. 253 (2), pp. 237-42. Date of Electronic Publication: 2005 Oct 10. - Publication Year :
- 2005
-
Abstract
- Pseudomonas putida vanillate-O-demethylase consisting of VanA and VanB was expressed in Escherichia coli strain K-12. Recombinant E. coli strain K-12 cells expressing VanAB efficiently converted vanillate into protocatechuate with glucose consumption. Mutant lacking either pgi or zwf showed higher or lower converting activity than the parental strain, respectively. Formaldehyde, which is the by-product of the demethylation, was converted into formate in the cellular reaction. Formate accumulation was blocked by gene disruption of the E. coli frmA that coded glutathione-dependent formaldehyde dehydrogenase.
- Subjects :
- DNA, Recombinant genetics
Escherichia coli genetics
Escherichia coli metabolism
Hydroxybenzoates metabolism
Methylation
NAD metabolism
NADP metabolism
Oxidoreductases, O-Demethylating genetics
Pseudomonas putida genetics
Vanillic Acid metabolism
Formaldehyde metabolism
Oxidoreductases, O-Demethylating metabolism
Pseudomonas putida enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1097
- Volume :
- 253
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 16242864
- Full Text :
- https://doi.org/10.1016/j.femsle.2005.09.036