Back to Search
Start Over
Selective decrease of chick embryonic primordial germ cells in vivo and in vitro by soft X-ray irradiation.
- Source :
-
Animal reproduction science [Anim Reprod Sci] 2006 Sep; Vol. 95 (1-2), pp. 67-74. Date of Electronic Publication: 2005 Oct 17. - Publication Year :
- 2006
-
Abstract
- The feasibility of soft (low-energy) X-ray irradiation as a means of depleting the endogenous primordial germ cell(s) (PGC) of chicken embryos, to improve the efficiency of germ cell-mediated transgenesis, was investigated. Eggs were subjected to a non-irradiated control treatment and embryos were exposed for 40s to soft X-ray at 15, 16.5, or 18 kV ( approximately 1.5, 1.65, and 1.8 Gy, respectively). Exposure of stage X embryos to each dose of X-ray resulted in a reduction of approximately 50% in the number of PGC apparent at stage 28, whereas the total number of gonadal cells was unaffected. Irradiation (16.5 kV) of embryos at stage 9 or 14 also resulted in similar decreases in the number of PGC with no effect on the total number of gonadal cells. Irradiation did not affect embryo hatchability, compared with the non-irradiated control treatment, although the hatch rate increased with the age of embryos at the time of irradiation. Exposure of gonadal cells isolated from stage 28 embryos to X-ray (16.5 kV, approximately 0.8 Gy) prevented the increase in PGC number during subsequent culture for 10 days; the increase in the total number of gonadal cells was not affected. In conclusion, exposure of chicken embryos to a low dose of soft X-rays is effective for depleting the endogenous PGC population without affecting embryo hatchability or somatic cell viability.
Details
- Language :
- English
- ISSN :
- 0378-4320
- Volume :
- 95
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Animal reproduction science
- Publication Type :
- Academic Journal
- Accession number :
- 16233964
- Full Text :
- https://doi.org/10.1016/j.anireprosci.2005.09.006