Back to Search
Start Over
Sugar-binding proteins potently inhibit dendritic cell human immunodeficiency virus type 1 (HIV-1) infection and dendritic-cell-directed HIV-1 transfer.
- Source :
-
Journal of virology [J Virol] 2005 Nov; Vol. 79 (21), pp. 13519-27. - Publication Year :
- 2005
-
Abstract
- Both endocytic uptake and viral fusion can lead to human immunodeficiency virus type 1 (HIV-1) transfer to CD4+ lymphocytes, either through directional regurgitation (infectious transfer in trans [I-IT]) or through de novo viral production in dendritic cells (DCs) resulting in a second-phase transfer to CD4+ lymphocytes (infectious second-phase transfer [I-SPT]). We have evaluated in immature monocyte-derived DCs both pathways of transfer with regard to their susceptibilities to being blocked by potential microbicidal compounds, including cyanovirin (CNV); the plant lectins Hippeastrum hybrid agglutinin, Galanthus nivalis agglutinin, Urtica dioica agglutinin, and Cymbidium hybrid agglutinin; and the glycan mannan. I-IT was a relatively inefficient means of viral transfer compared to I-SPT at both high and low levels of the viral inoculum. CNV was able to completely block I-IT at 15 microg/ml. All other compounds except mannan could inhibit I-IT by at least 90% when used at doses of 15 microg/ml. In contrast, efficient inhibition of I-SPT was remarkably harder to achieve, as 50% effective concentration levels for plant lectins and CNV to suppress this mode of HIV-1 transfer increased significantly. Thus, our findings indicate that I-SPT may be more elusive to targeting by antiviral drugs and stress the need for drugs affecting the pronounced inhibition of the infection of DCs by HIV-1.
- Subjects :
- Bacterial Proteins pharmacology
CD4-Positive T-Lymphocytes drug effects
CD4-Positive T-Lymphocytes virology
Carrier Proteins pharmacology
Coculture Techniques
Dendritic Cells drug effects
Dendritic Cells virology
Dose-Response Relationship, Drug
Galanthus
HIV-1 drug effects
Humans
Liliaceae
Urtica dioica
HIV Infections virology
HIV-1 growth & development
Plant Lectins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 79
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 16227272
- Full Text :
- https://doi.org/10.1128/JVI.79.21.13519-13527.2005