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Evaluation of immunity and protective efficacy of a dengue-3 pre-membrane and envelope DNA vaccine in Aotus nancymae monkeys.
- Source :
-
Vaccine [Vaccine] 2006 Feb 27; Vol. 24 (9), pp. 1427-32. Date of Electronic Publication: 2005 Sep 27. - Publication Year :
- 2006
-
Abstract
- A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.
- Subjects :
- Animals
Antibodies, Viral blood
Antigens, Viral genetics
Antigens, Viral immunology
Aotidae
Dengue Virus genetics
Dengue Virus immunology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Humans
Neutralization Tests
RNA, Viral blood
Vaccines, DNA administration & dosage
Viral Envelope Proteins genetics
Viral Vaccines administration & dosage
Dengue immunology
Dengue prevention & control
Vaccines, DNA immunology
Viral Envelope Proteins immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 24
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 16214271
- Full Text :
- https://doi.org/10.1016/j.vaccine.2005.09.032