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Comparison of three molecular methods used for subtyping of Legionella pneumophila strains isolated during an epidemic of Legionellosis in Rome.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2005 Oct; Vol. 43 (10), pp. 5348-50. - Publication Year :
- 2005
-
Abstract
- In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation among the isolates of the epidemic cluster. By comparison of the methods, SBT was the most rapid and the easiest to perform and provided unambiguous results.
- Subjects :
- Bacterial Proteins genetics
DNA, Bacterial analysis
Electrophoresis, Gel, Pulsed-Field
Humans
Italy epidemiology
Legionella pneumophila isolation & purification
Legionnaires' Disease microbiology
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Bacterial Typing Techniques
Disease Outbreaks
Legionella pneumophila classification
Legionella pneumophila genetics
Legionnaires' Disease epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 0095-1137
- Volume :
- 43
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16208015
- Full Text :
- https://doi.org/10.1128/JCM.43.10.5348-4350.2005