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Math1 expression redefines the rhombic lip derivatives and reveals novel lineages within the brainstem and cerebellum.
- Source :
-
Neuron [Neuron] 2005 Oct 06; Vol. 48 (1), pp. 31-43. - Publication Year :
- 2005
-
Abstract
- The rhombic lip (RL) is an embryonic proliferative neuroepithelium that generates several groups of hindbrain neurons. However, the precise boundaries and derivatives of the RL have never been genetically identified. We use beta-galactosidase expressed from the Math1 locus in Math1-heterozygous and Math1-null mice to track RL-derived cells and to evaluate their developmental requirements for Math1. We uncover a Math1-dependent rostral rhombic-lip migratory stream (RLS) that generates some neurons of the parabrachial, lateral lemniscal, and deep cerebellar nuclei, in addition to cerebellar granule neurons. A more caudal Math1-dependent cochlear extramural stream (CES) generates the ventral cochlear nucleus and cochlear granule neurons. Similarly, mossy-fiber precerebellar nuclei require Math1, whereas the inferior olive and locus coeruleus do not. We propose that Math1 expression delimits the extent of the rhombic lip and is required for the generation of the hindbrain superficial migratory streams, all of which contribute neurons to the proprioceptive/vestibular/auditory sensory network.
- Subjects :
- Age Factors
Animals
Basic Helix-Loop-Helix Transcription Factors deficiency
Basic Helix-Loop-Helix Transcription Factors genetics
Blotting, Western methods
Cell Movement physiology
Cochlea cytology
Cochlea embryology
Cochlea metabolism
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Embryo, Mammalian
Glycoside Hydrolases metabolism
Homeodomain Proteins genetics
Homeodomain Proteins metabolism
Immunohistochemistry methods
In Situ Hybridization methods
LIM-Homeodomain Proteins
Lac Operon physiology
Mice
Mice, Knockout
Models, Biological
RNA, Messenger biosynthesis
Reverse Transcriptase Polymerase Chain Reaction methods
T-Box Domain Proteins
Transcription Factors
Basic Helix-Loop-Helix Transcription Factors metabolism
Brain Stem cytology
Brain Stem embryology
Brain Stem metabolism
Cerebellum cytology
Cerebellum embryology
Cerebellum metabolism
Embryonic Development physiology
Gene Expression Regulation, Developmental physiology
Neurons physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0896-6273
- Volume :
- 48
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 16202707
- Full Text :
- https://doi.org/10.1016/j.neuron.2005.08.024