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Cell-free foetal DNA in maternal plasma does not appear to be derived from the rich pool of cell-free foetal DNA in amniotic fluid.
- Source :
-
Archives of gynecology and obstetrics [Arch Gynecol Obstet] 2006 Jan; Vol. 273 (4), pp. 221-6. Date of Electronic Publication: 2005 Sep 28. - Publication Year :
- 2006
-
Abstract
- Background: Large quantities of cell-free foetal DNA have been detected in amniotic fluid, and it has been proposed that this material may contribute to the pool of cell-free foetal DNA in maternal plasma.<br />Methods: Twelve maternal blood samples were obtained from pregnant women about to undergo an amniocentesis. Cell-free DNA was extracted from the maternal plasma samples and the matched amniotic fluid samples. The amount of cell-free foetal DNA was quantified by real-time PCR assays for the SRY and RHD genes.<br />Results: Amniotic fluid was found to contain vast quantities of cell-free DNA (median concentration = 3,978 copies/ml amniotic fluid). The concentration of cell-free foetal DNA in maternal plasma was much lower (median concentration = 96.6 copies/ml maternal plasma). No significant correlation could, however, be determined between these two pools of cell-free foetal DNA.<br />Conclusions: Our data confirm that amniotic fluid contains prodigious quantities of cell-free foetal DNA, but as no relationship exists between this material and that in the maternal circulation, it is unlikely that the amnion contributes to the presence of cell-free foetal DNA in maternal plasma.
Details
- Language :
- English
- ISSN :
- 0932-0067
- Volume :
- 273
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Archives of gynecology and obstetrics
- Publication Type :
- Academic Journal
- Accession number :
- 16189693
- Full Text :
- https://doi.org/10.1007/s00404-005-0068-0