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Priming-boosting vaccination with recombinant Mycobacterium bovis bacillus Calmette-Guérin and a nonreplicating vaccinia virus recombinant leads to long-lasting and effective immunity.
- Source :
-
Journal of virology [J Virol] 2005 Oct; Vol. 79 (20), pp. 12871-9. - Publication Year :
- 2005
-
Abstract
- Virus-specific T-cell responses can limit immunodeficiency virus type 1 (HIV-1) transmission and prevent disease progression and so could serve as the basis for an affordable, safe, and effective vaccine in humans. To assess their potential for a vaccine, we used Mycobacterium bovis bacillus Calmette-Guérin (BCG)-Tokyo and a replication-deficient vaccinia virus strain (DIs) as vectors to express full-length gag from simian immunodeficiency viruses (SIVs) (rBCG-SIVgag and rDIsSIVgag). Cynomolgus macaques were vaccinated with either rBCG-SIVgag dermally as a single modality or in combination with rDIsSIVgag intravenously. When cynomologus macaques were primed with rBCG-SIVgag and then boosted with rDIsSIVgag, high levels of gamma interferon (IFN-gamma) spot-forming cells specific for SIV Gag were induced. This combination regimen elicited effective protective immunity against mucosal challenge with pathogenic simian-human immunodeficiency virus for the 1 year the macaques were under observation. Antigen-specific intracellular IFN-gamma activity was similarly induced in each of the macaques with the priming-boosting regimen. Other groups receiving the opposite combination or the single-modality vaccines were not effectively protected. These results suggest that a recombinant M. bovis BCG-based vector may have potential as an HIV/AIDS vaccine when administered in combination with a replication-deficient vaccinia virus DIs vector in a priming-boosting strategy.
- Subjects :
- Animals
BCG Vaccine genetics
Cells, Cultured
Gene Products, gag genetics
Gene Products, gag immunology
Immunization, Secondary
Interferon-gamma immunology
Leukocytes, Mononuclear immunology
Macaca fascicularis
Male
SAIDS Vaccines administration & dosage
SAIDS Vaccines immunology
Simian Acquired Immunodeficiency Syndrome prevention & control
Simian Immunodeficiency Virus immunology
Smallpox Vaccine genetics
Species Specificity
Vaccines, Synthetic administration & dosage
BCG Vaccine administration & dosage
Genetic Vectors
Immunization Schedule
Simian Acquired Immunodeficiency Syndrome immunology
Smallpox Vaccine administration & dosage
Vaccination
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 79
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 16188989
- Full Text :
- https://doi.org/10.1128/JVI.79.20.12871-12879.2005