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Effect of treatment interruption monitored by CD4 cell count on mitochondrial DNA content in HIV-infected patients: a prospective study.
- Source :
-
AIDS (London, England) [AIDS] 2005 Oct 14; Vol. 19 (15), pp. 1627-33. - Publication Year :
- 2005
-
Abstract
- Background: HIV infection per se and HAART can alter mitochondrial functionality, leading to a decrease in mitochondrial DNA content.<br />Objective: To evaluate whether treatment interruption monitored by CD4 cell count can restore mitochondrial DNA content in peripheral blood lymphocytes.<br />Methods: Mitochondrial DNA content was measured in platelet-free CD4 and CD8 T cells by real-time polymerase chain reaction; flow cytometry was used to identify and quantify activated CD4 and CD8 T lymphocytes.<br />Results: The 30 patients had been treated for a mean of 107 months (range, 27-197). Median CD4 cell count at discontinuation was 702 cells/microl (range, 547-798). Median observational time from HAART discontinuation was 11.3 months (range, 4-26). Discontinuation of treatment provoked significant increases in mitochondrial DNA in CD8 T cells, which started only 6 months after therapy discontinuation [5.12 copies/cell per month from 0 to 6 months (P = 0.37) and 26.96 copies/cell per month from 6 to 12 months (P < 0.0001)].<br />Conclusions: This study is the first showing that mitochondrial DNA content can increase in peripheral blood lymphocytes during treatment interruption, but only after at least 6 months of interruption. Consequently, interruptions of shorter periods, whether by clinician or patient decision, are unlikely to allow restoration of mitochondrial DNA and so decrease HAART-related toxicity.
- Subjects :
- Adult
Anti-HIV Agents adverse effects
Antiretroviral Therapy, Highly Active adverse effects
Antiretroviral Therapy, Highly Active methods
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes drug effects
CD8-Positive T-Lymphocytes drug effects
DNA, Mitochondrial drug effects
Drug Administration Schedule
Drug Evaluation
Female
HIV Infections immunology
HIV Infections virology
Humans
Male
Middle Aged
Mitochondria drug effects
Mitochondria physiology
Polymerase Chain Reaction methods
Prospective Studies
Reverse Transcriptase Inhibitors adverse effects
Time Factors
Viral Load
Anti-HIV Agents administration & dosage
DNA, Mitochondrial blood
HIV Infections drug therapy
Reverse Transcriptase Inhibitors administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0269-9370
- Volume :
- 19
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 16184032
- Full Text :
- https://doi.org/10.1097/01.aids.0000186019.47297.0d