Indirect sandwich ELISA for antigen detection of African swine fever virus: comparison of polyclonal and monoclonal antibodies.

Authors :: Hutchings GH
Ferris NP
Source :: Journal of virological methods [J Virol Methods] 2006 Feb; Vol. 131 (2), pp. 213-7. Date of Electronic Publication: 2005 Sep 22.
Publication Year :: 2006
Abstract: Two indirect, sandwich ELISAs are described for use in African swine fever (ASF) diagnosis. One assay uses polyclonal serum raised in rabbits and guinea pigs against the cytoplasmic soluble ASF virus protein and the second, a combination of monoclonal antibody raised against the VP73 protein and rabbit polyclonal serum. Both assays have been shown to detect antigen of representative field strains of phylogenetically distinct groupings of ASF virus but the ELISA, which utilises polyclonal antisera was slightly more sensitive than that using the monoclonal antibody.

Subjects :: African Swine Fever Virus immunology
Animals
Antibodies, Monoclonal
Guinea Pigs
Rabbits
Sensitivity and Specificity
Swine
Viral Proteins analysis
African Swine Fever diagnosis
African Swine Fever Virus isolation & purification
Antigens, Viral analysis
Enzyme-Linked Immunosorbent Assay methods

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