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Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
- Source :
-
Current biology : CB [Curr Biol] 2005 Sep 20; Vol. 15 (18), pp. 1663-9. - Publication Year :
- 2005
-
Abstract
- Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180 functionally uncharacterized genes whose expression is upregulated during meiosis. Deletion of two genes, sgo1 and mde2, caused massive chromosome missegregation. sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination.
- Subjects :
- Chromatids genetics
Chromatids physiology
Chromosomal Proteins, Non-Histone genetics
DNA Primers
Forkhead Transcription Factors genetics
Gene Deletion
Gene Expression Profiling
Genetic Vectors
Green Fluorescent Proteins
Hygromycin B
Microscopy, Fluorescence
Polymerase Chain Reaction
Schizosaccharomyces pombe Proteins genetics
Streptothricins
Chromosomal Proteins, Non-Histone metabolism
Chromosome Segregation genetics
Forkhead Transcription Factors metabolism
Meiosis genetics
Schizosaccharomyces genetics
Schizosaccharomyces pombe Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0960-9822
- Volume :
- 15
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Current biology : CB
- Publication Type :
- Academic Journal
- Accession number :
- 16169489
- Full Text :
- https://doi.org/10.1016/j.cub.2005.07.059