Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China.

An aerobic, spore-forming, moderately halophilic bacterium, strain BH260(T), was isolated from a salt lake in China. Cells of this strain were found to be motile rods with flagella. The organism grew optimally at 30-32 degrees C and pH 8.0 in the presence of 12-15% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus. The levels of 16S rRNA gene sequence similarity for strain BH260(T) with respect to Lentibacillus salsus KCTC 3911(T), Lentibacillus juripiscarius JCM 12147(T) and Lentibacillus salicampi KCCM 41560(T) were 96.0, 95.5 and 95.3%, respectively. Chemotaxonomic data (a DNA G+C content of 44 mol%; MK-7 as the major isoprenoid quinone; A1gamma-meso-diaminopimelic acid as the cell-wall type; phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids; anteiso-C(15:0), anteiso-C(17:0) and iso-C(16:0) as the major fatty acids) also supported assignment of the isolate to the genus Lentibacillus. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus lacisalsi sp. nov. is proposed. The type strain is BH260(T) (=KCTC 3915(T)=DSM 16462(T)).