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In response to pathogens, glial cells dynamically and differentially regulate Toll-like receptor gene expression.
- Source :
-
Journal of neuroimmunology [J Neuroimmunol] 2005 Dec; Vol. 169 (1-2), pp. 116-25. Date of Electronic Publication: 2005 Sep 06. - Publication Year :
- 2005
-
Abstract
- The mechanisms that mediate innate immune recognition of CNS infections are unknown. This study provides a comparison of Toll-like receptor (TLR) gene expression in resting and virus infected CNS cells. N2a neuroblastoma cells expressed TLR 3 but demonstrated no change in TLR gene expression in response to either LPS or virus infection. N9 microglia and differentiated primary astrocytes expressed most TLR genes. TLR 2 expression was highest in N9 microglia and TLR 7 in astrocytes. In both glial cell types, LPS stimulation upregulated pro-inflammatory cytokines, TLR 2 and TLR 3 gene expression but down-regulated other TLR genes. RNA virus infection substantially increased levels of type-I interferon (IFN) and TLR 3 transcripts and to a lesser extent TLR 9 transcripts. Microglia and astrocytes thus have the ability to discriminate between pathogens and elicit an appropriate response.
- Subjects :
- Alphavirus Infections genetics
Animals
Animals, Newborn
Cells, Cultured
Mice
Mice, Inbred C57BL
Neuroblastoma
Neuroglia drug effects
Neuroglia metabolism
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction methods
Time Factors
Toll-Like Receptors classification
Alphavirus Infections metabolism
Gene Expression physiology
Lipopolysaccharides toxicity
Neuroglia virology
Semliki forest virus physiology
Toll-Like Receptors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0165-5728
- Volume :
- 169
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of neuroimmunology
- Publication Type :
- Academic Journal
- Accession number :
- 16146656
- Full Text :
- https://doi.org/10.1016/j.jneuroim.2005.08.006