Bioflavonoids and glutamine in diet and the Mg, Ca, Cu, Zn concentrations in heavy metals intoxicated rats' skins.

The effect of bioflavonoids on the Mg, Ca, Cu and Zn concentration in rats' skin intoxicated with heavy metals Pb or Cd. The animals were divided at random into nine groups, each of 6 rats. Group I obtained in drinking water the solution of plumbum nitrate, Pb(NO3)2 in concentration of 500 mg Pb/dm3, group II solution of lead ions--500 mg Pb/dm3 and quercetin--200 mg/dm3 and glutamine 4 g/dm3, group III--lead ions solution (500 mg Pb/dm3), catechin--200 mg/dm3 and glutamine 4 g/dm3, group IV received water solution of Pb(NO3)2--500mg Pb/dm3, naringenin--200 mg/dm3 and glutamine 4 g/dm3, V-VII groups obtained the same that groups I-IV, but instead of lead cadmium chloride (CdCl2) in the amount of 500 mg Cd/dm3 was used. Group IX was a control group, and these animals received redistilled water to drink. All the groups of rats were on a normal diet (LSM dry food) and they got solutions and food ad libitum. After the experiment, the animals were sacrificed under ketamine narcosis and while collecting tissues, the skins (together with furs) used for further studies were also obtained. Heavy metals intoxication caused a significant decrease of magnesium and calcium concentrations in the examined rats' skins, and bioflavonoids with glutamine dietary addition did not improve or improve to a small degree the levels of those elements. Lead, in opposition to cadmium, decreased zinc and copper levels in skins, and bioflavonoids with glutamine caused the return of those elements' concentrations to the values of control group.