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Expression of bioactive human M-CSF soluble receptor in transgenic tobacco plants.
- Source :
-
Protein expression and purification [Protein Expr Purif] 2006 Apr; Vol. 46 (2), pp. 367-73. Date of Electronic Publication: 2005 Aug 15. - Publication Year :
- 2006
-
Abstract
- The cDNA encoding N-terminal three immunoglobin-like domains of human M-CSFR was linked to His-tag and endoplasmic reticulum retention sequence (KDEL) before being inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, by Agrobacterium tumefaciens-mediated transformation. The insertion and expression of target gene were confirmed by PCR, ELISA, and Western blot. The recombinant M-CSFsR reached a maximum expression level of 1.92% of total soluble protein in transgenic tobacco plant leaf tissues. The recombinant M-CSFsR could be purified through a one-step IMAC process and its bioactivity was confirmed by the inhibition of colony formation of J6-1 cells. The results suggested that we successfully expressed a high level of bioactive human M-CSFsR in tobacco plants.
- Subjects :
- Cell Line
Humans
Plant Leaves genetics
Plant Leaves metabolism
Plants, Genetically Modified metabolism
Protein Structure, Tertiary genetics
Receptor, Macrophage Colony-Stimulating Factor genetics
Receptor, Macrophage Colony-Stimulating Factor isolation & purification
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins isolation & purification
Recombinant Fusion Proteins pharmacology
Nicotiana metabolism
Gene Expression
Plants, Genetically Modified genetics
Receptor, Macrophage Colony-Stimulating Factor biosynthesis
Recombinant Fusion Proteins biosynthesis
Nicotiana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1046-5928
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Protein expression and purification
- Publication Type :
- Academic Journal
- Accession number :
- 16139512
- Full Text :
- https://doi.org/10.1016/j.pep.2005.07.018