Back to Search
Start Over
Transgenic watermelon rootstock resistant to CGMMV (cucumber green mottle mosaic virus) infection.
- Source :
-
Plant cell reports [Plant Cell Rep] 2005 Aug; Vol. 24 (6), pp. 350-6. Date of Electronic Publication: 2005 Jun 14. - Publication Year :
- 2005
-
Abstract
- In watermelon, grafting of seedlings to rootstocks is necessary because watermelon roots are less viable than the rootstock. Moreover, commercially important watermelon varieties require disease-resistant rootstocks to reduce total watermelon yield losses due to infection with viruses such as cucumber green mottle mosaic virus (CGMMV). Therefore, we undertook to develop a CGMMV-resistant watermelon rootstock using a cDNA encoding the CGMMV coat protein gene (CGMMV-CP), and successfully transformed a watermelon rootstock named 'gongdae'. The transformation rate was as low as 0.1-0.3%, depending on the transformation method used (ordinary co-culture vs injection, respectively). However, watermelon transformation was reproducibly and reliably achieved using these two methods. Southern blot analysis confirmed that the CGMMV-CP gene was inserted into different locations in the genome either singly or multiple copies. Resistance testing against CGMMV showed that 10 plants among 140 T1 plants were resistant to CGMMV infection. This is the first report of the development by genetic engineering of watermelons resistant to CGMMV infection.
- Subjects :
- Agriculture methods
Capsid Proteins genetics
DNA, Complementary genetics
Gene Expression Regulation, Plant genetics
Gene Transfer Techniques
Plant Diseases genetics
Plant Diseases virology
Plant Roots genetics
Plant Roots virology
Transformation, Genetic genetics
Citrullus genetics
Citrullus virology
Immunity, Innate genetics
Plants, Genetically Modified genetics
Plants, Genetically Modified virology
Tobamovirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0721-7714
- Volume :
- 24
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 16136267
- Full Text :
- https://doi.org/10.1007/s00299-005-0946-8