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Quantification of immunoglobulin free light chains in cerebrospinal fluid by nephelometry.
- Source :
-
Journal of clinical immunology [J Clin Immunol] 2005 Jul; Vol. 25 (4), pp. 338-45. - Publication Year :
- 2005
-
Abstract
- Oligoclonal free light chains (FLC) banding has been described in multiple sclerosis (MS) and should be correlated with disease activity. However, discrepancies between studies have been reported because of differences in methods. A new quantitative, rapid, and automated method using nephelometry is now available. Our objective was to investigate the interest of this method for the diagnosis and prognosis of MS. For this purpose, FLC index was determined in paired samples of CSF and serum from consecutive and unselected patients from the same department of neurology. We enrolled 89 patients (33 MS, 15 "possible MS", and 41 controls) and correlated with IgG index, IgG oligoclonal banding, and clinical MS progression criteria. The main results were (1) FLC kappa index was more sensitive but less specific than IgG index for the diagnosis of MS, (2) two MS patients were negative for oligoclonal banding but exhibited a positive kappa index, (3) no relation between FLC kappa indices, MS clinical criteria, and disease progression was found. In conclusion, FLC kappa index should be considered as a useful complementary test for MS diagnosis. Its pronostic interest remains to be determined on a larger cohort of possible MS patients.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Disease Progression
Female
Humans
Immunoassay methods
Immunoglobulin G blood
Immunoglobulin G cerebrospinal fluid
Immunoglobulin Light Chains blood
Immunoglobulin kappa-Chains blood
Immunoglobulin kappa-Chains cerebrospinal fluid
Immunoglobulin lambda-Chains blood
Immunoglobulin lambda-Chains cerebrospinal fluid
Male
Middle Aged
Multiple Sclerosis diagnosis
Sensitivity and Specificity
Immunoglobulin Light Chains cerebrospinal fluid
Multiple Sclerosis cerebrospinal fluid
Nephelometry and Turbidimetry methods
Subjects
Details
- Language :
- English
- ISSN :
- 0271-9142
- Volume :
- 25
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16133990
- Full Text :
- https://doi.org/10.1007/s10875-005-5371-9