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Apoptotic signaling proteins: possible participation in the regulation of vasopressin and catecholamines biosynthesis in the hypothalamus.
- Source :
-
Histochemistry and cell biology [Histochem Cell Biol] 2005 Dec; Vol. 124 (6), pp. 523-33. Date of Electronic Publication: 2005 Aug 25. - Publication Year :
- 2005
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Abstract
- The role of apoptotic signaling proteins for long-lived neurons in the mature brain is poorly understood. Recently, we have shown that water deprivation leads to the activation of vasopressin (VP) secretion and expression of Bcl-2 and caspase-9 apototic proteins in the hypothalamus of the rat brain. In the present work, we continued to study a possible relationship between the functional activity of neurosecretory cells of the hypothalamus and apoptosis related proteins. We found that water deprivation leads to simultaneous activation of synthesis of VP and p53 and Bcl-2 apoptotic proteins in the mouse brain. To study a possible effect of apoptotic proteins on the functional state of hypothalamic neurons, the VP and tyrosine hydroxylase (TH) synthesis were analyzed in p53, p21(Waf1/Cip1) and Bcl-2 deficient mice. Loss of p53 and Bcl-2 significantly reduced VP synthesis in paraventricular and supraoptic nuclei and TH expression in arcuat, periventricular and zona incerta nuclei of the hypothalamus. Surprisingly, in contrast with the loss of p53, the inactivation of p21(Waf1/Cip1) up-regulates the expression of VP and TH. These data indicate that p53, p21(Waf1/Cip1) and Bcl-2 proteins, besides affecting cell cycle, tumor suppression and apoptosis, may act as modulators of neurosecretory activity of hypothalamic neurons; however, this problem remains to be determined more detailed.
- Subjects :
- Animals
Apoptosis physiology
Brain pathology
Brain ultrastructure
Cyclin-Dependent Kinase Inhibitor p21 deficiency
Gene Expression Regulation
Hypothalamus ultrastructure
Immunohistochemistry
In Situ Hybridization
Mice
Mice, Knockout
Proto-Oncogene Proteins c-bcl-2 deficiency
RNA, Messenger genetics
RNA, Messenger metabolism
Sensitivity and Specificity
Signal Transduction physiology
Tumor Suppressor Protein p53 deficiency
Catecholamines biosynthesis
Cyclin-Dependent Kinase Inhibitor p21 metabolism
Hypothalamus metabolism
Proto-Oncogene Proteins c-bcl-2 metabolism
Tumor Suppressor Protein p53 metabolism
Vasopressins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0948-6143
- Volume :
- 124
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Histochemistry and cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 16133124
- Full Text :
- https://doi.org/10.1007/s00418-005-0016-x