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Chromosome 16-specific repetitive DNA sequences that map to chromosomal regions known to undergo breakage/rearrangement in leukemia cells.
- Source :
-
Genomics [Genomics] 1992 Jun; Vol. 13 (2), pp. 332-8. - Publication Year :
- 1992
-
Abstract
- Human chromosome 16-specific low-abundance repetitive (CH16LAR) DNA sequences have been identified during the course of constructing a physical map of this chromosome. At least three CH16LAR sequences exist and they are interspersed, in small clusters, over four regions that constitute more than 5% of the chromosome. CH16LAR sequences were observed in one unusually large cosmid contig (number 55), where the ordering of clones was difficult because these sequences led to false overlaps between noncontiguous clones. Contig 55 contains 78 clones, or approximately 2% of all the clones contained within the present cosmid contig physical map. Fluorescent in situ hybridization of multiple clones, including cosmid and YAC contig 55 clones, mapped the four CH16LAR-rich regions to bands p13, p12, p11, and q22. These regions are of biological interest since the pericentric inversion and the interhomologue translocation breakpoints commonly found in acute nonlymphocytic leukemia (ANLL) subtype M4 fall within these bands. Sequence analysis of a 2.2-kb HindIII fragment from a cosmid containing a CH16LAR sequence indicated that one of the CH16LAR elements is similar to a minisatellite sequence in that the core repeat is only 40 bp in length. Additional characterization of other repetitive elements is in progress.
- Subjects :
- Animals
Base Sequence
Blotting, Southern
Chickens genetics
Chromosome Aberrations
Chromosome Inversion
Chromosome Mapping
Gene Rearrangement
Humans
Leukemia, Myelomonocytic, Acute genetics
Mammals genetics
Molecular Sequence Data
Neoplasms genetics
Nucleic Acid Hybridization
Restriction Mapping
Saccharomyces cerevisiae genetics
Chromosomes, Human, Pair 16
Repetitive Sequences, Nucleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 0888-7543
- Volume :
- 13
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 1612592
- Full Text :
- https://doi.org/10.1016/0888-7543(92)90249-r