Back to Search
Start Over
MALT1 contains nuclear export signals and regulates cytoplasmic localization of BCL10.
- Source :
-
Blood [Blood] 2005 Dec 15; Vol. 106 (13), pp. 4210-6. Date of Electronic Publication: 2005 Aug 25. - Publication Year :
- 2005
-
Abstract
- MALT1, BCL10 (B-cell lymphoma 10), and API2 (apoptosis inhibitor 2)-MALT1 are key molecules in mucosa-associated lymphoid tissue (MALT) lymphomagenesis. We previously reported that MALT1 and API2-MALT1 were localized only in cytoplasm, where we suggested that both molecules were likely to be active. In the study presented here, we further examined the localization-determining region by generating various mutants and were able to demonstrate that there were nuclear export signal (NES)-containing domains in the MALT1 C-terminal region. The use of leptomycin B, an NES-specific inhibitor, demonstrated that both MALT1 and API2-MALT1 were predominantly retained in the nuclei, indicating that these molecules were shuttling between nucleus and cytoplasm in an NES-dependent manner. It was also found that MALT1 was involved in the nuclear export of BCL10, which is originally localized in both nucleus and cytoplasm. These results correlate well with the nuclear BCL10 expression pattern in both t(1;14) and t(11;18) MALT lymphomas. The nucleocytoplasmic shuttling of MALT1 and BCL10 complex may indicate that these molecules are involved not only in the nuclear factor kappaB (NF-kappaB) pathway but also in other biologic functions in lymphocytes.
- Subjects :
- Amino Acid Sequence
Animals
Apoptosis Regulatory Proteins genetics
Apoptosis Regulatory Proteins metabolism
B-Cell CLL-Lymphoma 10 Protein
COS Cells
Caspases
Chlorocebus aethiops
Humans
Lymphoma, B-Cell, Marginal Zone genetics
Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
NF-kappa B metabolism
Neoplasm Proteins genetics
Protein Transport
Sequence Homology, Amino Acid
Adaptor Proteins, Signal Transducing metabolism
Cytoplasm metabolism
Lymphoma, B-Cell, Marginal Zone metabolism
Neoplasm Proteins metabolism
Nuclear Export Signals physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 106
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 16123224
- Full Text :
- https://doi.org/10.1182/blood-2004-12-4785