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CpG-oligodeoxynucleotide protects immune cells from gamma-irradiation-induced cell death.
- Source :
-
Molecular immunology [Mol Immunol] 2006 Mar; Vol. 43 (8), pp. 1163-71. Date of Electronic Publication: 2005 Aug 24. - Publication Year :
- 2006
-
Abstract
- Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by gamma-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from gamma-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.
- Subjects :
- Animals
B-Lymphocytes immunology
Cell Death radiation effects
Cell Survival
Dose-Response Relationship, Radiation
Humans
Lymphocyte Subsets drug effects
Lymphocyte Subsets radiation effects
Mice
Proto-Oncogene Proteins c-bcl-2 genetics
Spleen cytology
Spleen drug effects
Spleen radiation effects
Time Factors
Up-Regulation genetics
bcl-X Protein genetics
B-Lymphocytes drug effects
B-Lymphocytes radiation effects
Gamma Rays
Oligodeoxyribonucleotides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0161-5890
- Volume :
- 43
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16122803
- Full Text :
- https://doi.org/10.1016/j.molimm.2005.07.020