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RNA interference against Hec1 inhibits tumor growth in vivo.
- Source :
-
Gene therapy [Gene Ther] 2006 Jan; Vol. 13 (1), pp. 1-7. - Publication Year :
- 2006
-
Abstract
- Hec1 (highly expressed in cancer) plays an important role in chromosome segregation by interacting with a subset of checkpoint proteins that survey proper chromosome alignment and bipolar spindle attachment. In order to disrupt mitotic progression of tumor cell lines, we have used retrovirus and adenovirus vectors that inhibit Hec1 synthesis. Vector-expressed short hairpin RNAs (shRNAs) caused very efficient depletion of the target protein, cellular arrest and considerable mitotic catastrophe induction 96 h post infection in human cervix-adenocarcinoma (HeLa) and glioblastoma (U-373-MG) cell lines. Furthermore, adenocarcinomas induced in the flanks of nude mice show significant reduction in size compared with control when treated with either Hec1-shRNA retroviruses or adenoviruses. These results indicate that depletion of Hec1 could be used as a new strategy to block the dividing cell, and therefore against cancer.
- Subjects :
- Adenocarcinoma pathology
Adenocarcinoma therapy
Adenoviridae genetics
Animals
Brain Neoplasms pathology
Brain Neoplasms therapy
Chromosome Segregation drug effects
Cytoskeletal Proteins
Female
Flow Cytometry
Genetic Vectors genetics
Genetic Vectors therapeutic use
Glioblastoma pathology
Glioblastoma therapy
HeLa Cells
Humans
Mice
Mice, Nude
Microscopy, Fluorescence
Mitosis drug effects
Neoplasm Transplantation
Neoplasms pathology
Retroviridae genetics
Uterine Cervical Neoplasms pathology
Uterine Cervical Neoplasms therapy
Genetic Therapy methods
Neoplasms therapy
Nuclear Proteins genetics
RNA Interference
RNA, Small Interfering genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0969-7128
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gene therapy
- Publication Type :
- Academic Journal
- Accession number :
- 16121206
- Full Text :
- https://doi.org/10.1038/sj.gt.3302595