[The acquisition of two silkworm CAPs markers and their use in genetic diversity and phylogenetic relationship].

Cleaved amplified polymorphic sequence (CAPs) markers are based on PCR amplification of known genes, cDNA sequences or RAPD sequences. The PCR products are digested by restriction enzymes, generating the simple type of data as heterozygotes and homozygotes. Here we designed primers based on silkworm attacin and alpha-amylase genes, then digested the PCR products in silkworm strains P50, C108 and their progeny F1 using 4 different restriction enzymes respectively. Furthermore, the genetic diversity and phylogenetic relationship of 12 silkworm strains were investigated using the obtained two CAPs markers.