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Mouse anterior pituitary gland: analysis by ion trap mass spectrometry.
- Source :
-
Neuroendocrinology [Neuroendocrinology] 2005; Vol. 81 (4), pp. 229-43. Date of Electronic Publication: 2005 Aug 10. - Publication Year :
- 2005
-
Abstract
- We investigated the proteome of the anterior pituitary gland (AP) in a species in which the genome has been sequenced. Subcellular fractions of APs from 2-month-old male mice were prepared for protein denaturation, treatment with trypsin and analyses utilizing micro liquid chromatography tandem mass spectrometry and the database search software SEQUEST. In the nuclear, non-nuclear 100,000 g and cytosolic fractions, we identified 49, 36 and 68 different proteins, respectively. A total of 115 distinct proteins were detected. We identified growth hormone, prolactin, pro-opiomelanocortin, the alpha-subunit for the glycoprotein hormones, and luteinizing hormone-beta. Groups of other identified proteins included hormone-processing, secretion granule-associated, non-hormonal endoplasmic reticulum-associated, calcium-binding, protein kinase C-associated, histones, non-histone chromosomal, other RNA-binding, heterogeneous nuclear ribonucleoproteins, splicing factors, helicases, lamins, ribosomal, microtubule-associated, microfilament-associated, adenosine triphosphate- and guanosine triphosphate-associated, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation, enzymes in glycolysis and the tricarboxylic and urea cycles and the pentose phosphate path, heat-shock, glutathione-associated, peroxidases, ubiquitin-associated, catabolic, protease inhibitors, other, and blood proteins. The 115 proteins reported in this study and the 145 proteins reported in a previous study on the AP of the adult male Golden Syrian hamster are compared and form a foundation for defining the proteome in normal adult male AP.<br /> (Copyright 2005 S. Karger AG, Basel.)
- Subjects :
- Animals
Cell Nucleus chemistry
Chromatography, Liquid
Cricetinae
Cytosol chemistry
Male
Mesocricetus
Mice
Pituitary Gland, Anterior ultrastructure
Pituitary Hormones analysis
Protein Denaturation
Software
Trypsin metabolism
Mass Spectrometry methods
Pituitary Gland, Anterior chemistry
Proteome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0028-3835
- Volume :
- 81
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Neuroendocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 16103733
- Full Text :
- https://doi.org/10.1159/000087434