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Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2005 Sep 23; Vol. 280 (38), pp. 32640-8. Date of Electronic Publication: 2005 Jul 27. - Publication Year :
- 2005
-
Abstract
- Screening of cdc mutants of fission yeast for those whose cell cycle arrest is independent of the DNA damage checkpoint identified the RNA splicing-deficient cdc28 mutant. A search for mutants of cdc28 cells that enter mitosis with unspliced RNA resulted in the identification of an orb5 point mutant. The orb5+ gene, which encodes a catalytic subunit of casein kinase II, was found to be required for cell cycle arrest in other mutants with defective RNA metabolism but not for operation of the DNA replication or DNA damage checkpoints. Loss of function of wee1+ or rad24+ also suppressed the arrest of several splicing mutants. Overexpression of the major B-type cyclin Cdc13p induced cdc28 cells to enter mitosis. The abundance of Cdc13p was reduced, and the phosphorylation of Cdc2p on tyrosine 15 was maintained in splicing-defective cells. These results suggest that regulation of Cdc13p and Cdc2p is required for G2 arrest in splicing mutants.
- Subjects :
- Alternative Splicing
Blotting, Northern
CDC2 Protein Kinase genetics
CDC28 Protein Kinase, S cerevisiae genetics
Casein Kinase II metabolism
Cell Cycle
Cell Cycle Proteins genetics
Cell Line
Cell Survival
Cyclin B genetics
DNA metabolism
DNA Damage
G2 Phase
Genotype
Immunoblotting
Intracellular Signaling Peptides and Proteins genetics
Mitosis
Mutation
Nuclear Proteins genetics
Phosphorylation
Point Mutation
Protein-Tyrosine Kinases genetics
RNA metabolism
Schizosaccharomyces pombe Proteins genetics
Schizosaccharomyces pombe Proteins metabolism
Time Factors
CDC2 Protein Kinase biosynthesis
Cyclin B biosynthesis
Gene Expression Regulation, Fungal
RNA Splicing
Schizosaccharomyces genetics
Schizosaccharomyces pombe Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 280
- Issue :
- 38
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16049013
- Full Text :
- https://doi.org/10.1074/jbc.M504746200