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Determination of nevirapine and efavirenz in plasma using GC/MS in selected ion monitoring mode.
- Source :
-
Therapeutic drug monitoring [Ther Drug Monit] 2005 Aug; Vol. 27 (4), pp. 521-5. - Publication Year :
- 2005
-
Abstract
- An accurate, selective, and sensitive method for the determination of the nonnucleoside reverse transcriptase inhibitors (NNRTIs) nevirapine (nvp) and efavirenz (efv) in human plasma using gas chromatography-mass spectroscopy in selected ion monitoring mode (GC/MS-SIM) was developed. Solid-phase extraction (SPE) gave extraction yields near 100% for both nvp and efv, and calibration curves were linear over the therapeutic concentration ranges. Variation of intraday and interday precision was below 5%. Intraday and interday inaccuracies varied between 0.6% and 10.4%. The lower limits of detection using a 200-microL plasma sample were 27 ng/mL for nvp and 26 ng/mL for efv, and the lower limits of quantification were 54 ng/mL and 72 ng/mL, respectively. The method was applied to the determination of nvp and efv in plasma specimens of 73 patients in HIV stage III or IV and on antiretroviral treatment in Kigali, Rwanda.
- Subjects :
- Alkynes
Anti-HIV Agents blood
Anti-HIV Agents therapeutic use
Antiretroviral Therapy, Highly Active methods
Benzoxazines
Calibration
Cyclopropanes
Dose-Response Relationship, Drug
Drug Monitoring methods
HIV Infections drug therapy
Humans
Ions blood
Ions chemistry
Lamivudine blood
Lamivudine therapeutic use
Molecular Structure
Nevirapine chemistry
Nevirapine therapeutic use
Oxazines chemistry
Oxazines therapeutic use
Reproducibility of Results
Gas Chromatography-Mass Spectrometry methods
Nevirapine blood
Oxazines blood
Subjects
Details
- Language :
- English
- ISSN :
- 0163-4356
- Volume :
- 27
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Therapeutic drug monitoring
- Publication Type :
- Academic Journal
- Accession number :
- 16044111
- Full Text :
- https://doi.org/10.1097/01.ftd.0000164613.91555.2e