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Analysis of pregabalin at therapeutic concentrations in human plasma/serum by reversed-phase HPLC.
- Source :
-
Therapeutic drug monitoring [Ther Drug Monit] 2005 Aug; Vol. 27 (4), pp. 451-6. - Publication Year :
- 2005
-
Abstract
- A selective and sensitive HPLC method is described for assay of the new antiepileptic drug pregabalin in serum/plasma. Following acetone precipitation of the sample, the drug was derivatized with picryl sulfonic acid (PSA) before chromatography on a C8 column. No interference from coadministered antiepileptics has been observed. The assay is calibrated over the range 0.5 mg/L to 8 mg/L. In addition to qualitative and quantitative validation data for the assay, concentration measurements in predose samples from a group of patients with dose escalated to 600 mg/d pregabalin are presented. The drug concentrations measured were in the range 2.8-8.2 mg/L at steady state. The method is robust and will be suitable for monitoring pregabalin therapy.
- Subjects :
- Anticonvulsants blood
Anticonvulsants pharmacokinetics
Anticonvulsants therapeutic use
Calibration
Carbamazepine blood
Carbamazepine pharmacokinetics
Carbamazepine therapeutic use
Chromatography, High Pressure Liquid instrumentation
Dose-Response Relationship, Drug
Drug Interactions
Drug Stability
Drug Therapy, Combination
Epilepsy blood
Epilepsy drug therapy
Humans
Lamotrigine
Pregabalin
Reproducibility of Results
Spectrophotometry, Ultraviolet methods
Triazines blood
Triazines pharmacokinetics
Triazines therapeutic use
Trinitrobenzenesulfonic Acid chemistry
Valproic Acid blood
Valproic Acid pharmacokinetics
Valproic Acid therapeutic use
gamma-Aminobutyric Acid blood
gamma-Aminobutyric Acid pharmacokinetics
gamma-Aminobutyric Acid therapeutic use
Chromatography, High Pressure Liquid methods
gamma-Aminobutyric Acid analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0163-4356
- Volume :
- 27
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Therapeutic drug monitoring
- Publication Type :
- Academic Journal
- Accession number :
- 16044101
- Full Text :
- https://doi.org/10.1097/01.ftd.0000158874.54100.1a