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Glucose trimming of N-glycan in endoplasmic reticulum is indispensable for the growth of Raphanus sativus seedling (kaiware radish).
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2005 Jul; Vol. 69 (7), pp. 1353-64. - Publication Year :
- 2005
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Abstract
- Recently I found that glycosidase inhibitors such as castanospermine, deoxynojirimycin, swainsonine, 2-acetamindo 2,3-dideoxynojirimycin, and deoxymannojirimycin change the N-glycan structure of root glycoproteins, and that the glucosidase inhibitors castanospermine and deoxynojirimycin suppress the growth of Raphanus sativus seedlings (Mega, T., J. Biochem., 2004). The present study undertook to see whether the growth suppression is due to the inhibition of glucose trimming in endoplasmic reticulum (ER). The study, using three glucosidase inhibitors, castanospermine, N-methyl deoxynojirimycin, and deoxynojirimycin, upon the growth of R. sativus foliage leaf, made clear that glucose trimming is indispensable for plant growth, because the inhibition of glucose trimming correlated with leaf growth. On the other hand, processing inhibition in the Golgi apparatus by other glycosidase inhibitors had little effect on plant growth, although N-glycan processing was disrupted depending on inhibitor specificity. These results suggest that N-glycan processing after glucosidase processing is dispensable for plant growth and cell differentiation.
- Subjects :
- 1-Deoxynojirimycin pharmacology
Amino Sugars pharmacology
Chromatography, High Pressure Liquid methods
Dose-Response Relationship, Drug
Enzyme Inhibitors pharmacology
Glucose analysis
Glycoside Hydrolases antagonists & inhibitors
Glycoside Hydrolases metabolism
Golgi Apparatus metabolism
Indolizines pharmacology
Models, Biological
Oligosaccharides analysis
Oligosaccharides metabolism
Plant Leaves drug effects
Plant Leaves growth & development
Plant Leaves metabolism
Raphanus chemistry
Raphanus growth & development
Rhamnose analogs & derivatives
Rhamnose pharmacology
Seedlings drug effects
Seedlings growth & development
Swainsonine pharmacology
Endoplasmic Reticulum metabolism
Glucose metabolism
Polysaccharides metabolism
Raphanus metabolism
Seedlings metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0916-8451
- Volume :
- 69
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 16041142
- Full Text :
- https://doi.org/10.1271/bbb.69.1353