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Anti-metabolic activity of caspofungin against Candida albicans and Candida parapsilosis biofilms.
- Source :
-
The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2005 Sep; Vol. 56 (3), pp. 507-12. Date of Electronic Publication: 2005 Jul 22. - Publication Year :
- 2005
-
Abstract
- Objectives: Candidiasis can be associated with the formation of biofilms on bioprosthetic surfaces and the intrinsic resistance of Candida albicans biofilms to the most commonly used antifungal agents has been demonstrated. In this study, we report on the antifungal activity of caspofungin at two different concentrations, on C. albicans and Candida parapsilosis biofilms with different ages of maturation.<br />Methods: Fifteen strains of C. albicans (10 strains susceptible to fluconazole in vitro and five strains resistant to this antifungal agent) and six strains of C. parapsilosis (all were susceptible to fluconazole in vitro) were studied. The antifungal activity of caspofungin was assessed by looking for a significant inhibition of the metabolic activity of yeasts within biofilms. Biofilms of Candida were produced in vitro, on silicone catheters.<br />Results: Caspofungin used at MIC did not modify the metabolic activity of C. albicans, whatever the maturation age of the biofilms. The same concentration of caspofungin significantly reduced the metabolism (P<or=0.001) of 25% (biofilms of 48 h) to 50% (biofilms of 2 h) of the C. parapsilosis yeasts. The use of a therapeutic concentration of caspofungin (2 mg/L) significantly decreased (P<or=0.001) the metabolism of all the strains of C. albicans and C. parapsilosis tested, independently of the biofilm maturation age. This potent antifungal activity of caspofungin on C. albicans biofilms was observed independently of the yeast susceptibility to fluconazole.<br />Conclusions: This study demonstrated that caspofungin used at MIC was not sufficient to reduce C. albicans biofilms, but it suggested an activity on C. parapsilosis biofilms depending on their maturation age. This study also indicated that caspofungin used at 2 mg/L could be a good candidate in the prevention of candidiasis associated with silicone medical devices. Our results also suggested that fluconazole resistance of yeasts did not affect caspofungin activity.
- Subjects :
- Biofilms growth & development
Candida physiology
Candida albicans
Caspofungin
Dose-Response Relationship, Drug
Drug Resistance, Multiple, Fungal
Echinocandins
Fluconazole pharmacology
Lipopeptides
Microbial Sensitivity Tests
Antifungal Agents pharmacology
Antimetabolites pharmacology
Biofilms drug effects
Candida drug effects
Peptides, Cyclic pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0305-7453
- Volume :
- 56
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of antimicrobial chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 16040622
- Full Text :
- https://doi.org/10.1093/jac/dki269