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Photoactivation of hypericin generates singlet oxygen in mitochondria and inhibits succinoxidase.
- Source :
-
Photochemistry and photobiology [Photochem Photobiol] 1992 Jan; Vol. 55 (1), pp. 47-53. - Publication Year :
- 1992
-
Abstract
- Photosensitized inhibition of mitochondrial succinoxidase by hypericin was measured in vitro and found to be drug-dose, light-dose, and wavelength dependent. Singlet oxygen generation, monitored using the singlet oxygen trap tetramethylethylene, and oxygen consumption in isolated mitochondria sensitized by hypericin were also light-dose and wavelength dependent. Unequivocal evidence for the generation of singlet oxygen was obtained using kinetic isotope ratios of products from the reaction between singlet oxygen and geminally deuterated tetramethylethylene. An action spectrum for the inhibition of succinoxidase was measured at wavelengths between 400 and 700 nm and found to parallel the recorded visible absorption spectrum of hypericin in isolated mitochondria. The greatest singlet oxygen generation, oxygen consumption, and succinoxidase inhibition occurred with white light or 600 nm irradiation. These data are consistent with a type II singlet-oxygen-mediated mechanism for hypericin induced photosensitized inhibition of mitochondrial succinoxidase.
- Subjects :
- Animals
Anthracenes
Cattle
Light
Mitochondria, Heart drug effects
Mitochondria, Heart radiation effects
Perylene pharmacology
Singlet Oxygen
Mitochondria, Heart metabolism
Oxidoreductases antagonists & inhibitors
Oxygen metabolism
Perylene analogs & derivatives
Radiation-Sensitizing Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0031-8655
- Volume :
- 55
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Photochemistry and photobiology
- Publication Type :
- Academic Journal
- Accession number :
- 1603850
- Full Text :
- https://doi.org/10.1111/j.1751-1097.1992.tb04208.x