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Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes.
- Source :
-
Genes and immunity [Genes Immun] 2005 Oct; Vol. 6 (7), pp. 588-95. - Publication Year :
- 2005
-
Abstract
- Gene expression profiles permit analysis of host immune response at the transcriptome level. We used the Pax gene Blood RNA (PAX) System and Affymetrix microarrays (HG-U133A&B) to survey profiles in basic military trainees and to classify them as healthy, febrile respiratory illness (FRI) without adenovirus, FRI with adenovirus, and convalescent from FRI with adenovirus. We assessed quality metrics of RNA processing for microarrays. Class prediction analysis discovered nested sets of transcripts that could categorize the phenotypes with optimized accuracy of 99% (nonfebrile vs febrile, P<0.0005), 87% (healthy vs convalescent, P=0.001), and 91% (febrile without vs with adenovirus, P<0.0005). The discovered set for classification of nonfebrile vs febrile patients consisted of 40 transcripts with functions related to interferon induced genes, complement cascades, and TNF and IL1 signaling. The set of seven transcripts for distinguishing healthy vs convalescent individuals included those associated with ribosomal structure, humoral immunity, and cell adhesion. The set of 10 transcripts for distinguishing FRI without vs with adenovirus had functions related to interferon induced genes, IL1 receptor accessory protein, and cell interactions. These results are the first in vivo demonstration of classification of infectious diseases via host signature transcripts and move us towards using the transcriptome in bio-surveillance.<br /> (Genes and Immunity (2005) 6, 588-595. doi:10.1038/sj.gene.6364244; published online 21 July 2005.)
- Subjects :
- Adenovirus Infections, Human diagnosis
Adenoviruses, Human
Antibody Formation genetics
Cell Adhesion genetics
Convalescence
Gene Expression Regulation
Humans
Male
Oligonucleotide Array Sequence Analysis
Phenotype
Respiratory Tract Infections diagnosis
Respiratory Tract Infections virology
Transcription, Genetic
Adenovirus Infections, Human classification
Gene Expression Profiling
Military Personnel
Respiratory Tract Infections classification
Subjects
Details
- Language :
- English
- ISSN :
- 1466-4879
- Volume :
- 6
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Genes and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 16034474
- Full Text :
- https://doi.org/10.1038/sj.gene.6364244