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Isolation and characterization of a new Mycobacterium austroafricanum strain, IFP 2015, growing on MTBE.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2006 Apr; Vol. 70 (3), pp. 358-65. Date of Electronic Publication: 2005 Jul 19. - Publication Year :
- 2006
-
Abstract
- A new Mycobacterium austroafricanum strain, IFP 2015, growing on methyl tert-butyl ether (MTBE) as a sole carbon source was isolated from an MTBE-degrading microcosm inoculated with drain water of an MTBE-supplemented gasoline storage tank. M. austroafricanum IFP 2015 was able to grow on tert-butyl formate, tert-butyl alcohol (TBA) and alpha-hydroxyisobutyrate. 2-Methyl-1,2-propanediol was identified as the TBA oxidation product in M. austroafricanum IFP 2015 and in the previously isolated M. austroafricanum IFP 2012. M. austroafricanum IFP 2015 also degraded ethyl tert-butyl ether more rapidly than M. austroafricanum IFP 2012. Specific primers designed to monitor the presence of M. austroafricanum strains could be used as molecular tools to detect similar strains in MTBE-contaminated environment.
- Subjects :
- Bacterial Proteins genetics
Chaperonin 60
Chaperonins genetics
Culture Media
DNA, Bacterial analysis
DNA, Ribosomal analysis
Fresh Water chemistry
Molecular Sequence Data
Mycobacterium classification
Mycobacterium genetics
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Fresh Water microbiology
Gasoline
Methyl Ethers metabolism
Mycobacterium growth & development
Mycobacterium isolation & purification
Water Pollutants, Chemical metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0175-7598
- Volume :
- 70
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 16028043
- Full Text :
- https://doi.org/10.1007/s00253-005-0074-y