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Identification of Escherichia coli K12 YdcW protein as a gamma-aminobutyraldehyde dehydrogenase.
- Source :
-
FEBS letters [FEBS Lett] 2005 Aug 01; Vol. 579 (19), pp. 4107-12. - Publication Year :
- 2005
-
Abstract
- Gamma-aminobutyraldehyde dehydrogenase (ABALDH) from wild-type E. coli K12 was purified to apparent homogeneity and identified as YdcW by MS-analysis. YdcW exists as a tetramer of 202+/-29 kDa in the native state, a molecular mass of one subunit was determined as 51+/-3 kDa. Km parameters of YdcW for gamma-aminobutyraldehyde, NAD+ and NADP+ were 41+/-7, 54+/-10 and 484+/-72 microM, respectively. YdcW is the unique ABALDH in E. coli K12. A coupling action of E. coli YgjG putrescine transaminase and YdcW dehydrogenase in vitro resulted in conversion of putrescine into gamma-aminobutyric acid.
- Subjects :
- Aldehyde Oxidoreductases chemistry
Aldehyde Oxidoreductases isolation & purification
Amino Acid Sequence
Chromatography, High Pressure Liquid
Escherichia coli enzymology
Escherichia coli Proteins chemistry
Escherichia coli Proteins isolation & purification
Molecular Sequence Data
Sequence Homology, Amino Acid
Aldehyde Oxidoreductases metabolism
Escherichia coli metabolism
Escherichia coli Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 579
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 16023116
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.06.038