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The solution structure of the Josephin domain of ataxin-3: structural determinants for molecular recognition.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2005 Jul 26; Vol. 102 (30), pp. 10493-8. Date of Electronic Publication: 2005 Jul 14. - Publication Year :
- 2005
-
Abstract
- The Josephin domain plays an important role in the cellular functions of ataxin-3, the protein responsible for the neurodegenerative Machado-Joseph disease. We have determined the solution structure of Josephin and shown that it belongs to the family of papain-like cysteine proteases, sharing the highest degree of structural similarity with bacterial staphopain. A currently unique structural feature of Josephin is a flexible helical hairpin formed by a 32-residue insertion, which could determine substrate specificity. By using the Josephin structure and the availability of NMR chemical shift assignments, we have mapped the enzyme active site by using the typical cysteine protease inhibitors, transepoxysuccinyl-L-eucylamido-4-guanidino-butane (E-64) and [L-3-trans-(propylcarbamyl)oxirane-2-carbonyl]-L-isoleucyl-L-proline (CA-074). We also demonstrate that the specific interaction of Josephin with the ubiquitin-like domain of the ubiquitin- and proteasome-binding factor HHR23B involves complementary exposed hydrophobic surfaces. The structural similarity with other deubiquitinating enzymes suggests a model for the proteolytic enzymatic activity of ataxin-3.
- Subjects :
- Ataxin-3
DNA Repair Enzymes
DNA-Binding Proteins
Dipeptides
Escherichia coli
Leucine analogs & derivatives
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular
Nuclear Proteins
Protein Structure, Tertiary
Repressor Proteins
Substrate Specificity
Machado-Joseph Disease enzymology
Models, Molecular
Nerve Tissue Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 102
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 16020535
- Full Text :
- https://doi.org/10.1073/pnas.0501732102