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Differential coronary microvascular exchange responses to adenosine: roles of receptor and microvessel subtypes.
- Source :
-
Microcirculation (New York, N.Y. : 1994) [Microcirculation] 2005 Jun; Vol. 12 (4), pp. 313-26. - Publication Year :
- 2005
-
Abstract
- Objective: To assess the role of adenosine receptors in the regulation of coronary microvascular permeability to porcine serum albumin (P(s)(PSA)).<br />Methods: Solute flux was measured in single perfused arterioles and venules isolated from pig hearts using fluorescent dye-labeled probes by microspectro-fluorometry. Messenger RNA, protein, and cellular distribution of adenosine receptors in arterioles and venules were analyzed by RT-PCR, immunoblot, and immunofluorescence.<br />Results: Control venule P(s)(PSA) (10.7 +/- 4.8 x 10(- 7) cm x s(- 1)) was greater than that of arterioles (6.4+/- 2.8 x 10(-7) cm . s(-1); p < .05). Arteriolar P(s)(PSA) decreased (p < .05) with adenosine suffusion over the range from 10(- 8) to 10(-5) M, while venular P(s)(PSA) did not change. The nonselective A(1) and A(2) receptor antagonist, 8-(p-sulfophenyl) theophylline, blocked the adenosine-induced decrease in arteriolar P(s)(PSA). Messenger RNA for adenosine A(1), A(2A), A(2B), and A(3) receptors was expressed in arterioles and venules. Protein for A(1), A(2A), and A(2B), but not A(3), was detected in both microvessel types and was further demonstrated on vascular endothelial cells.<br />Conclusion: Arteriolar P(s)(PSA) decreases with adenosine suffusion but not venular P(s)(PSA). Adenosine A(1), A(2A), and A(2B) receptors are expressed in both arterioles and venules. Selective receptor-linked cellular signaling mechanisms underlying the regulation of permeability remain to be determined.
- Subjects :
- Adenosine pharmacology
Animals
Arterioles chemistry
Fluorescent Dyes
In Vitro Techniques
Microcirculation
Microscopy, Fluorescence
Perfusion
RNA, Messenger analysis
Receptor, Adenosine A1 analysis
Receptor, Adenosine A1 genetics
Receptor, Adenosine A1 physiology
Receptor, Adenosine A2A analysis
Receptor, Adenosine A2A genetics
Receptor, Adenosine A2A physiology
Receptor, Adenosine A2B analysis
Receptor, Adenosine A2B genetics
Receptor, Adenosine A2B physiology
Receptor, Adenosine A3 analysis
Receptor, Adenosine A3 genetics
Receptor, Adenosine A3 physiology
Receptors, Purinergic P1 analysis
Receptors, Purinergic P1 genetics
Serum Albumin metabolism
Swine
Venules chemistry
Capillary Permeability
Coronary Circulation physiology
Receptors, Purinergic P1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1073-9688
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microcirculation (New York, N.Y. : 1994)
- Publication Type :
- Academic Journal
- Accession number :
- 16020078
- Full Text :
- https://doi.org/10.1080/10739680590934736