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Expression of cellular FLICE/caspase-8 inhibitory protein is associated with malignant potential in endometrial carcinoma.
- Source :
-
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society [Int J Gynecol Cancer] 2005 Jul-Aug; Vol. 15 (4), pp. 663-70. - Publication Year :
- 2005
-
Abstract
- This study aimed to investigate the expression of cellular Fas-associated death domain-like interleukin-1beta-converting enzyme (FLICE)/caspase-8 inhibitory protein (c-FLIP) in endometrial carcinoma and its possible implications. c-FLIP protein was detected in 42 endometrial carcinoma tissues and in 22 normal proliferative endometrial tissues by immunohistochemistry. In addition, c-FLIP messenger ribonucleic acid (mRNA) was evaluated in 20 endometrial carcinomas and in 18 normal proliferative endometria by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) using SYBR Green I(TM). The relationship between c-FLIP protein level and tumor cell proliferation and that between c-FLIP protein level and clinicopathologic parameters of patients with endometrial carcinoma was analyzed. c-FLIP protein expression was significantly higher in neoplastic tissues than in normal tissues (P < 0.01), and similar result was obtained from RT-PCR analysis of c-FLIP mRNA (P < 0.01). Furthermore, c-FLIP protein was significantly associated with proliferating cell nuclear antigen-labeling index (P < 0.01), clinical stage (P < 0.05), the presence of invasion to > 1/2 myometrium (P < 0.05), and lymph node metastasis (P < 0.01). Multivariate analysis of variance also confirmed the association of c-FLIP with clinical stage (P < 0.05) and with lymph node metastasis (P < 0.05), while its association with myometrial invasion was marginal (P = 0.059). It is concluded that c-FLIP might contribute to the carcinogenesis and aggressiveness of endometrial carcinoma and might be a useful prognostic factor in the tumor.
- Subjects :
- Adult
CASP8 and FADD-Like Apoptosis Regulating Protein
Case-Control Studies
Cell Proliferation
Cell Transformation, Neoplastic
Endometrial Neoplasms genetics
Female
Gene Expression Profiling
Humans
Immunohistochemistry
Intracellular Signaling Peptides and Proteins genetics
Middle Aged
Neoplasm Invasiveness
Prognosis
Proliferating Cell Nuclear Antigen metabolism
RNA, Messenger analysis
RNA, Messenger biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Endometrial Neoplasms metabolism
Endometrial Neoplasms pathology
Intracellular Signaling Peptides and Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1048-891X
- Volume :
- 15
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
- Publication Type :
- Academic Journal
- Accession number :
- 16014121
- Full Text :
- https://doi.org/10.1111/j.1525-1438.2005.00122.x