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[Recombinant design and expression of human three-domain antibody against BoNTa].
- Source :
-
Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2005 Apr; Vol. 45 (2), pp. 223-5. - Publication Year :
- 2005
-
Abstract
- VH and Vkappa genes were amplified from human ScFv B17 specific against botulinum neurotoxin serotype A (BoNTa). Gene sequence encoding 5'-terminal 12 amino acid of heavy chain constant region CH1, as a linker, linked VH and Vkappa to construct a new three-domain antibody molecule VH/Vkappa. VH/Vkappa was expressed at high level over 34% of total host cell proteins in E. coli. Recombinant protein were purified up to 95% by affinity column. As a result, recombinant VH/Vkappa could recognize and bind specific to BoNTa in ELISA. However, comparing with its parent ScFv, VH/Vkappa has higher relative affinity and stability.
- Subjects :
- Antibodies, Monoclonal chemistry
Antibodies, Monoclonal genetics
Antibodies, Monoclonal immunology
Antibody Specificity
Escherichia coli genetics
Humans
Immunoglobulin Fragments chemistry
Immunoglobulin Heavy Chains chemistry
Immunoglobulin Heavy Chains genetics
Immunoglobulin Light Chains chemistry
Immunoglobulin Light Chains genetics
Immunoglobulin Variable Region chemistry
Immunoglobulin Variable Region genetics
Recombinant Proteins chemistry
Recombinant Proteins immunology
Antibodies, Monoclonal biosynthesis
Botulinum Toxins, Type A immunology
Immunoglobulin Fragments genetics
Recombinant Proteins biosynthesis
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0001-6209
- Volume :
- 45
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Wei sheng wu xue bao = Acta microbiologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 15989265