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Development and optimization of a method for the analysis of Brazilein by HPLC with electrochemical detection.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2005 Jun 03; Vol. 1077 (1), pp. 44-8. - Publication Year :
- 2005
-
Abstract
- The aim of this study was to establish an easy and accurate method for the determination of Brazilein in plant samples due to its potential pharmacological activities. High-performance liquid chromatography (HPLC) with electrochemical detection (ED) was used for the assay of Brazilein in this study for the first time. Crucial influence parameters including concentration of dodecane-1-sulfonic acid sodium salt (DSASS), inorganic modifier, tetrabutyl-ammonium hydroxide solution (TBAOH), and applied potential of proposed method were investigated. The proposed method is simple, rapid (analysis time: approximately 10 min), sensitive [(detection limit: 0.6 ng per injection (20 microl) at a signal-noise ratio 3:1)], highly selective and precise (intra- and inter-day precisions were within 5%, n = 7). The calibration graph of Brazilein was linear in the range 0.6-150 ng per injection 20 microl. Recovery of Brazilein was over 92% by standard addition method.
Details
- Language :
- English
- ISSN :
- 0021-9673
- Volume :
- 1077
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 15988985
- Full Text :
- https://doi.org/10.1016/j.chroma.2005.04.050