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[Evaluation of seven immunological assay reagents for hepatitis B virus surface antigen (HBsAg) in the sensitivity and the detection of HBsAg mutants].
- Source :
-
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases [Kansenshogaku Zasshi] 2005 Feb; Vol. 79 (2), pp. 138-42. - Publication Year :
- 2005
-
Abstract
- Mutants of hepatitis B virus surface antigen (HBsAg) are known as a cause of false-negative results in diagnostic tests for HBsAg; particularly when a diagnostic kit utilizes monoclonal antibodies to detect HBsAg. We compared seven HBsAg kits with regard to sensitivity for HBsAg subtypes (ad, ay) and their ability to detect nine different HBsAg mutants. Among them, the sensitivities of five kits were high and comparable to each other (0.2 - 0.3ng/ml). However, two kits were of lower sensitivity (0.8-1.3ng/ml, and 2.4-2.5ng/ml, respectively). Two kits, produced by the same company, reacted with all of the nine HBsAg mutants, but five kits showed false-negative results with one or more of the HBsAg mutants. These data indicate that there are differences in the detection sensitivities for HBsAg and abilities to detect HBsAg mutants among commercially available HBsAg kits, which may explain false-negative clinical results.
- Subjects :
- Antibodies, Monoclonal
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
False Negative Reactions
Hepatitis B Surface Antigens genetics
Immunoassay methods
Sensitivity and Specificity
Hepatitis B Surface Antigens isolation & purification
Hepatitis B virus genetics
Reagent Kits, Diagnostic standards
Subjects
Details
- Language :
- Japanese
- ISSN :
- 0387-5911
- Volume :
- 79
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 15977551
- Full Text :
- https://doi.org/10.11150/kansenshogakuzasshi1970.79.138