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Joint development in Xenopus laevis and induction of segmentations in regenerating froglet limb (spike).
- Source :
-
Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2005 Aug; Vol. 233 (4), pp. 1444-53. - Publication Year :
- 2005
-
Abstract
- In Xenopus laevis, amputation of the adult limb results in the formation of a simple (hypomorphic) spike-like structure without joints, although tadpole limb bud regenerates complete limb pattern. The expression of some joint marker genes was examined in limb development and regeneration. Bmp-4 and gdf-5 were expressed and sox-9 expression was decreased in the joint region. Although developing cartilages were well-organized and had bmp-4 expressing perichondrocytes, the spike cartilage did not have such a structure, but only showed sparse bmp-4 expression. Application of BMP4-soaked beads to the spike led to the induction of a joint-like structure. These results suggest that the lack of joints in the spike is due to the deficiency of the accumulation of the cells that express bmp-4. Improvement of regeneration in the Xenopus adult limb that we report here for the first time will give us important insights into epimorphic regeneration.<br /> ((c) 2005 Wiley-Liss, Inc.)
- Subjects :
- Animals
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins biosynthesis
Bone Morphogenetic Proteins genetics
Cartilage physiology
Extremities growth & development
Growth Differentiation Factor 5
High Mobility Group Proteins biosynthesis
High Mobility Group Proteins genetics
Immunohistochemistry
Molecular Probes
SOX9 Transcription Factor
Transcription Factors biosynthesis
Transcription Factors genetics
Triazines
Xenopus Proteins
Extremities physiology
Joints physiology
Regeneration physiology
Xenopus laevis
Subjects
Details
- Language :
- English
- ISSN :
- 1058-8388
- Volume :
- 233
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Developmental dynamics : an official publication of the American Association of Anatomists
- Publication Type :
- Academic Journal
- Accession number :
- 15977182
- Full Text :
- https://doi.org/10.1002/dvdy.20484