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Brussels sprouts, inulin and fermented milk alter the faecal microbiota of human microbiota-associated rats as shown by PCR-temporal temperature gradient gel electrophoresis using universal, Lactobacillus and Bifidobacterium 16S rRNA gene primers.
- Source :
-
The British journal of nutrition [Br J Nutr] 2005 May; Vol. 93 (5), pp. 677-84. - Publication Year :
- 2005
-
Abstract
- We investigated the effect of Brussels sprouts, inulin and a fermented milk on the faecal microbiota diversity of human microbiota-associated (HMA) rats by PCR-temporal temperature gradient gel electrophoresis (PCR-TTGE) using universal and group-specific 16S rRNA gene primers. The HMA rats were submitted to a control diet for 10 d (initial time), then switched to the experimental diets for 4 weeks (final time). Using universal primers, the mean degree of similarity between all faecal samples at initial time was 80.8 %. In the group consuming the control diet throughout the experiment, the mean degree of similarity between the PCR-TTGE profiles at initial v. final time was 76.8 %, reflecting a spontaneous temporal variation. The mean degree of similarity between control and experimental groups at final time was lower, 72.4 %, 74.4 % and 75.6 % for inulin, Brussels sprouts and fermented milk, respectively, indicating a dietary effect on the predominant populations. Using specific primers, bifidobacteria could be detected only in those rats that had consumed inulin, showing a specific increasing effect of this dietary compound. The Lactobacillus population was very heterogeneous at initial time but tended to homogenize within each dietary group. At final time, caecal contents were collected for analysis of SCFA and beta-glucuronidase activity. Inulin and Brussels sprouts increased the butyrate and acetate proportion, respectively, while the fermented milk did not modify the caecal biochemistry. This experiment shows for the first time that cruciferous vegetables are able to alter the diversity and the metabolic activities of the digestive microbiota in HMA rats.
- Subjects :
- Acetates analysis
Animals
Brassica
Butyrates analysis
Cecum
Cultured Milk Products
Electrophoresis, Polyacrylamide Gel
Fatty Acids, Volatile analysis
Feces chemistry
Glucuronidase analysis
Humans
Intestines microbiology
Inulin administration & dosage
Male
Polymerase Chain Reaction
Rats
Rats, Inbred F344
Bifidobacterium genetics
Diet
Feces microbiology
Lactobacillus genetics
RNA, Bacterial analysis
RNA, Ribosomal, 16S analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0007-1145
- Volume :
- 93
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 15975167
- Full Text :
- https://doi.org/10.1079/bjn20051372