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Influence of disaccharide structure on prebiotic selectivity in vitro.
Influence of disaccharide structure on prebiotic selectivity in vitro.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2005 Jun 29; Vol. 53 (13), pp. 5192-9. - Publication Year :
- 2005
-
Abstract
- To obtain structure-function information of a range of carbohydrates, which are available only in very small quantities, an in vitro fermentation method using 7 mg of carbohydrate, 0.7 mL of basal medium, and 1% (w/v) of fecal bacteria was validated against a pH-controlled batch culture with 150 mL of basal medium and 1.5 g of test carbohydrate. This method was used to determine the influence of different glycosidic linkages and monosaccharide compositions of disaccharides on the selectivity of microbial fermentation. A prebiotic index (PI) was calculated for each disaccharide. Generally, disaccharides with linkages of 1-2, 1-4, and 1-6 generated a high PI score, with kojibiose and sophorose showing the greatest values (21.62 and 18.63, respectively). Apart from 6alpha-mannobiose, mannose-containing disaccharides gave a low PI due to low numbers of bifidobacteria and lactobacilli and an increase in bacteroides. The structure-function information obtained in this study may lead to a predictive understanding of how specific structures are fermented by the human gut microflora.
Details
- Language :
- English
- ISSN :
- 0021-8561
- Volume :
- 53
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15969496
- Full Text :
- https://doi.org/10.1021/jf050276w