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1H-N.m.r. study of enzymically generated wheat-endosperm arabinoxylan oligosaccharides: structures of hepta- to tetradeca-saccharides containing two or three branched xylose residues.
- Source :
-
Carbohydrate research [Carbohydr Res] 1992 Jan; Vol. 223, pp. 19-44. - Publication Year :
- 1992
-
Abstract
- The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1----4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, ----4)[alpha-L-Araf-(1----3)]-beta-D-Xylp-(1---- and/or ----4) [alpha-L-Araf-(1----2)][alpha-L-Araf-(1----3)]-beta-D-Xyl p-(1----, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established.
- Subjects :
- Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Ion Exchange
Glycoside Hydrolases
Hydrogen
Magnetic Resonance Spectroscopy methods
Models, Structural
Molecular Sequence Data
Oligosaccharides isolation & purification
Seeds
Spectrometry, Mass, Fast Atom Bombardment
Triticum
Xylan Endo-1,3-beta-Xylosidase
Arabinose analysis
Oligosaccharides chemistry
Xylose analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0008-6215
- Volume :
- 223
- Database :
- MEDLINE
- Journal :
- Carbohydrate research
- Publication Type :
- Academic Journal
- Accession number :
- 1596918
- Full Text :
- https://doi.org/10.1016/0008-6215(92)80003-j