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Integration of mutations responsible for the attenuated phenotype of Pepper mild mottle virus strains results in a symptomless cross-protecting strain.
- Source :
-
Archives of virology [Arch Virol] 2005 Oct; Vol. 150 (10), pp. 2009-20. Date of Electronic Publication: 2005 Jun 22. - Publication Year :
- 2005
-
Abstract
- An enhanced attenuated strain of Pepper mild mottle virus (PMMoV) was constructed by incorporating mutations that affect viral attenuation from three reported attenuated strains of PMMoV, which causes serious economic losses in the production of green pepper in Japan. The new strain caused no symptoms on pepper plants and protected them from infection by a wild-type strain. The mutations responsible for viral attenuation were located in the intervening region (IR) of the 126-kDa/183-kDa proteins. The mutations had synergistic effects in terms of the attenuation of symptoms and decreased the accumulation of the viral coat protein in infected pepper plants. In this paper, we propose an efficient method for the improvement of attenuated viruses by reverse genetics in plant viruses.
- Subjects :
- Base Sequence
Capsid Proteins genetics
Capsid Proteins metabolism
DNA, Complementary genetics
DNA, Viral genetics
Genes, Viral
Japan
Molecular Sequence Data
Mutation
Phenotype
Plant Diseases virology
Plant Viral Movement Proteins
Tobamovirus physiology
Viral Proteins genetics
Viral Proteins metabolism
Virulence genetics
Capsicum virology
Tobamovirus genetics
Tobamovirus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 0304-8608
- Volume :
- 150
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 15968477
- Full Text :
- https://doi.org/10.1007/s00705-005-0551-8