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Ovarian brain-derived neurotrophic factor (BDNF) promotes the development of oocytes into preimplantation embryos.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2005 Jun 28; Vol. 102 (26), pp. 9206-11. Date of Electronic Publication: 2005 Jun 20. - Publication Year :
- 2005
-
Abstract
- Optimal development of fertilized eggs into preimplantation embryos is essential for reproduction. Although mammalian oocytes ovulated after luteinizing hormone (LH) stimulation can be fertilized and promoted into early embryos in vitro, little is known about ovarian factors important for the conditioning of eggs for early embryo development. Because LH interacts only with ovarian somatic cells, its potential regulation of oocyte functions is presumably mediated by local paracrine factors. We performed DNA microarray analyses of ovarian transcripts and identified brain-derived neurotrophic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the preovulatory LH surge. Ovarian BDNF acts on TrkB receptors expressed exclusively in oocytes to enhance first polar body extrusion of oocytes and to promote the in vitro development of zygotes into preimplantation embryos. Furthermore, in vivo treatment with a Trk receptor inhibitor suppressed first polar body extrusion and the progression of zygotes into blastocysts. Thus, ovarian BDNF is important to nuclear and cytoplasmic maturation of the oocyte, which is essential for successful oocyte development into preimplantation embryos. Treatment with BDNF could condition the cultured oocytes for optimal progression into the totipotent blastocysts.
- Subjects :
- Animals
Blastocyst metabolism
Cell Nucleus metabolism
Cytoplasm metabolism
Female
Fertilization
Gonadotropins metabolism
Luteinizing Hormone metabolism
Mice
Microscopy, Fluorescence
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Ovarian Follicle metabolism
Ovulation
Receptor, Nerve Growth Factor
Receptor, trkB metabolism
Receptors, Nerve Growth Factor metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Brain-Derived Neurotrophic Factor metabolism
Oocytes metabolism
Ovary metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 102
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 15967989
- Full Text :
- https://doi.org/10.1073/pnas.0502442102