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In vitro interaction between surface-treated Ti-6Al-4V titanium alloy and human peripheral blood mononuclear cells.
- Source :
-
Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2005 Aug 01; Vol. 74 (2), pp. 197-207. - Publication Year :
- 2005
-
Abstract
- The Ti-6Al-4V titanium alloy is widely employed as an implant material. The effects of Ti-6Al-4V samples, tested in both an untreated state and one in which the samples were subjected to a glow-discharge treatment performed with the use of air, on human peripheral blood mononuclear cells (PBMC) were studied. Apoptosis, undetectable after 24-h contact of PBMC with the two sample types, is induced after 48 h by treated samples, and, after 48 h, but in the presence of 1.5 microg/mL PHA, by both sample types. The expression of intercellular adhesion molecule-1 (ICAM-1) always increases, in comparison with control, in PBMC put in contact with the two sample types. In the same way, a remarkable increase in tumor necrosis-alpha (TNF-alpha) release in the culture medium is registered, when PBMC are put in contact with the two sample types for 24 and 48 h. Human umbilical-vein endothelial cells (HUVEC) cocultured for 48 h with PBMC, previously incubated with the two sample types for 24 h, show an increase in ICAM-1 and vascular cell adhesion molecule-1 (VCAM-1) protein expression in comparison with control (HUVEC cocultured with control PBMC), indicating that inflammatory phenomena might occur. Taken together, these results suggest that, although plasma-treated titanium alloy shows a better biocompatibility in comparison with the untreated one, attention must be paid to the careful control of the first signs of inflammation.<br /> ((c) 2005 Wiley Periodicals, Inc.)
Details
- Language :
- English
- ISSN :
- 1549-3296
- Volume :
- 74
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of biomedical materials research. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 15965911
- Full Text :
- https://doi.org/10.1002/jbm.a.30366