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Trafficking of STEVOR to the Maurer's clefts in Plasmodium falciparum-infected erythrocytes.
- Source :
-
The EMBO journal [EMBO J] 2005 Jul 06; Vol. 24 (13), pp. 2306-17. Date of Electronic Publication: 2005 Jun 16. - Publication Year :
- 2005
-
Abstract
- The human malarial parasite Plasmodium falciparum exports proteins to destinations within its host erythrocyte, including cytosol, surface and membranous profiles of parasite origin termed Maurer's clefts. Although several of these exported proteins are determinants of pathology and virulence, the mechanisms and trafficking signals underpinning protein export are largely uncharacterized-particularly for exported transmembrane proteins. Here, we have investigated the signals mediating trafficking of STEVOR, a family of transmembrane proteins located at the Maurer's clefts and believed to play a role in antigenic variation. Our data show that, apart from a signal sequence, a minimum of two addition signals are required. This includes a host cell targeting signal for export to the host erythrocyte and a transmembrane domain for final sorting to Maurer's clefts. Biochemical studies indicate that STEVOR traverses the secretory pathway as an integral membrane protein. Our data suggest general principles for transport of transmembrane proteins to the Maurer's clefts and provide new insights into protein sorting and trafficking processes in P. falciparum.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, Protozoan genetics
Erythrocyte Membrane metabolism
Erythrocyte Membrane parasitology
Erythrocytes parasitology
Green Fluorescent Proteins genetics
Host-Parasite Interactions
Humans
Membrane Proteins genetics
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Sorting Signals
Protein Transport
Recombinant Fusion Proteins metabolism
Antigens, Protozoan metabolism
Erythrocytes metabolism
Membrane Proteins metabolism
Plasmodium falciparum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0261-4189
- Volume :
- 24
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- The EMBO journal
- Publication Type :
- Academic Journal
- Accession number :
- 15961998
- Full Text :
- https://doi.org/10.1038/sj.emboj.7600720