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Mice vaccinated with allergen-pulsed myeloid dendritic cells are not protected from developing allergen-induced Th2 responses.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2005 Jul; Vol. 137 (3), pp. 219-28. Date of Electronic Publication: 2005 Jun 13. - Publication Year :
- 2005
-
Abstract
- Background: Dendritic cells (DC) play a decisive role in the induction of allergen-induced Th1 and Th2 responses. Since the induction of allergen-specific Th1 responses has shown to inhibit allergen-induced Th2-type inflammation, in this study we investigated whether manipulated myeloid-derived DC pulsed with the specific allergen would predominantly induce allergen-specific Th1 responses thereby reducing the development of Th2 responses.<br />Methods: Murine bone marrow (BM)-DC were generated and pulsed with ovalbumin (OVA) and CpG oligodeoxynucleotides (CpG-ODN). Langerhans cells (LC) were also isolated and pulsed in vitro with OVA. Subsequently, mice were vaccinated intravenously with either CpG/OVA-pulsed BM-DC or OVA-pulsed LC, and the protocol to induce OVA-specific Th2 responses using OVA/alum sensitization was initiated. Airway inflammation and OVA-specific serum antibody levels were evaluated 6 days after the intranasal challenge with OVA.<br />Results: The application ofCpG/OVA-pulsed BM-DC was unable to reduce airway eosinophilia and inflammation in OVA/alum-immunized mice. OVA-specific IgG1 or IgE serum levels were also not reduced. The experiments using LC pulsed with OVA yielded similar results. However, mice vaccinated with CpG/OVA-pulsed BM-DC had greatly enhanced levels of serum OVA-specific IgG2a, suggesting the induction of allergen-specific Th1 responses in vivo. Moreover, allergen-induced mast cell degranulation was decreased using this approach.<br />Conclusions: Taken together, our results demonstrated that the vaccination with OVA-pulsed BM-DC matured with CpG-ODN or OVA-pulsed LC did not result in a reduction in allergen-specific Th2 responses in a murine model of severe atopic asthma. Other DC-based vaccination strategies should be evaluated in order to prevent the development of allergic disorders.<br /> (Copyright 2005 S. Karger AG, Basel)
- Subjects :
- Allergens administration & dosage
Animals
Base Sequence
Cytokines metabolism
Eosinophilia therapy
Female
Immunoglobulin E biosynthesis
Immunoglobulin E blood
Immunoglobulin G biosynthesis
Immunoglobulin G blood
Mice
Molecular Sequence Data
Myeloid Cells immunology
Oligodeoxyribonucleotides administration & dosage
Oligodeoxyribonucleotides immunology
Ovalbumin administration & dosage
Ovalbumin immunology
Vaccines administration & dosage
Allergens immunology
Dendritic Cells immunology
Th2 Cells immunology
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1018-2438
- Volume :
- 137
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15956790
- Full Text :
- https://doi.org/10.1159/000086334