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ACE2, CBK1, and BUD4 in budding and cell separation.
- Source :
-
Eukaryotic cell [Eukaryot Cell] 2005 Jun; Vol. 4 (6), pp. 1018-28. - Publication Year :
- 2005
-
Abstract
- Mutations in the RAM network genes, including CBK1, MOB2, KIC1, HYM1, and TAO3, cause defects in bud site selection, asymmetric apical growth, and mating projections. Additionally, these mutants show altered colony morphology, cell separation defects, and reduced CTS1 expression, phenotypes also seen by mutating the Ace2 transcription factor. We show that an ACE2 multicopy plasmid suppresses the latter three defects of RAM network mutations, demonstrating that Ace2 is downstream of the RAM network and suggesting that these phenotypes are caused by reduced expression of Ace2 target genes. We show that wild-type W303 strains have a bud4 mutation and that combining bud4 with either ace2 or cbk1 in haploids results in altered colony morphology. We describe a timed sedimentation assay that allows quantitation of cytokinesis defects and subtle changes in budding pattern and cell shape. Experiments examining budding patterns and sedimentation rates both show that Ace2 and Cbk1 have independent functions in addition to their common pathway in transcription of genes such as CTS1. SWI5 encodes a transcription factor paralogous to ACE2. Additive effects are seen in cbk1 swi5 strains, and we show that activation of some target genes, such as EGT2, requires either Swi5 or Ace2 with Cbk1. The relative roles and interactions of Ace2, Cbk1, and Bud4 in bud site selection, polarized growth, and cell separation are discussed.
- Subjects :
- Cell Cycle Proteins genetics
DNA-Binding Proteins genetics
Diploidy
Fungal Proteins genetics
GTP-Binding Proteins genetics
Haploidy
Intracellular Signaling Peptides and Proteins
Mutation
Protein Serine-Threonine Kinases
Saccharomyces cerevisiae cytology
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae growth & development
Saccharomyces cerevisiae physiology
Saccharomyces cerevisiae Proteins genetics
Transcription Factors genetics
Transcription, Genetic
Cell Cycle Proteins physiology
Cytokinesis
DNA-Binding Proteins physiology
Fungal Proteins physiology
GTP-Binding Proteins physiology
Gene Expression Regulation, Fungal
Saccharomyces cerevisiae Proteins physiology
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1535-9778
- Volume :
- 4
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Eukaryotic cell
- Publication Type :
- Academic Journal
- Accession number :
- 15947194
- Full Text :
- https://doi.org/10.1128/EC.4.6.1018-1028.2005